RT Journal Article
SR Electronic
T1 Insecticide susceptibility of <em>Aedes (Stegomyia) aegypti</em> (Linnaeus, 1762) and <em>Aedes (Stegomyia) albopictus</em> (Skuse, 1894) in Kinshasa, Democratic Republic of the Congo
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.11.08.467678
DO 10.1101/2021.11.08.467678
A1 Fabien Vulu
A1 Gillon Ilombe
A1 Lucrecia Vizcaino
A1 Joachim Mariën
A1 Yasue Morimoto
A1 David Weetman
A1 Audrey Lenhart
A1 Seth R. Irish
A1 Thierry L. Bobanga
YR 2021
UL http://biorxiv.org/content/early/2021/11/11/2021.11.08.467678.abstract
AB Aedes aegypti and Aedes albopictus are arbovirus vectors of public health concern. Although the Democratic Republic of the Congo (DRC) faces a long-standing risk of Aedes-borne viruses, data on insecticide resistance of Aedes populations are absent. To address this gap, we investigated insecticide susceptibility of Ae. aegypti and Ae. albopictus in areas with a high risk of arbovirus transmission. We also investigated the frequency of knock-down resistance (kdr) mutations in Ae. aegypti. Immature stages of Ae. aegypti and Ae. albopictus were collected from two sites in Kinshasa (Lingwala and Cité Verte) between April and July 2017 and reared to the adult stage. Wild-caught adult Ae. aegypti were collected in 2016 in another site (Ngaliema). Female Ae. aegypti (from Lingwala) and Ae. albopictus (from Cité Verte) were used in WHO tube insecticide susceptibility tests. The F1534C, V1016I and V410L kdr mutations were genotyped in Ae. aegypti from Lingwala and Ngaliema. We observed Ae. aegypti to be susceptible to bendiocarb, propoxur and malathion, suspected resistant to permethrin, and resistant to deltamethrin and DDT. Aedes albopictus was susceptible to bendiocarb, propoxur, malathion and permethrin, suspected resistant to deltamethrin and resistant to DDT. While F1534C and V1016I were not detected, a few Ae. aegypti from Lingwala were heterozygous for the mutation V410L. This study reports for the first time the insecticide resistance status of Aedes spp. and the detection of the kdr mutation V410L in Ae. aegypti in DRC. Given the resistance profile, carbamates and potentially malathion are recommended insecticide options against Ae. aegypti in Kinshasa. It will be important to develop Aedes control strategies based on the resistance patterns of Aedes in Kinshasa.Competing Interest StatementThe authors have declared no competing interest.