RT Journal Article
SR Electronic
T1 Autologous adipose-derived stem cell transplantation enhances healing of wound with exposed bone in a rat model
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 571968
DO 10.1101/571968
A1 Tomo Hamada
A1 Hidenori Matsubara
A1 Yasuhisa Yoshida
A1 Shuhei Ugaji
A1 Issei Nomura
A1 Hiroyuki T suchiya
YR 2019
UL http://biorxiv.org/content/early/2019/03/08/571968.abstract
AB Objectives Soft tissue wounds with exposed bone often require extended healing times and can be associated with severe complications. We describe the ability of artificial dermis with autogenic adipose-derived stem cells (ADSCs) to promote the healing of wounds with exposed bone in a rat model.Methods Adipose tissues harvested from the bilateral inguinal regions of Wistar rats were used as ADSCs. Rats were randomly divided into control and ADSC groups to investigate the efficacy of ADSC transplantation for wound healing (n=20 per group). Soft tissue defects were created on the heads of the rats and were covered with artificial dermis with or without the seeded ADSCs. Specimens from these rats were evaluated using digital image analysis, histology, immunohistochemistry, cell labeling, and real-time reverse-transcription polymerase chain reaction (Real-time RT-PCR).Results The average global wound area was significantly smaller in the ADSC group than in the control group on days 3, 7, and 14 after surgery (p<0.05). After 14 days, the blood vessel density in the wound increased by 1.6-fold in the ADSC group compared with that in the control group (p<0.01). Real-time RT-PCR results showed higher Fgfb and Vegf expression levels at all time points, and higher Tgfb1 and Tgfb3 expression levels until 14 days after surgery, in the ADSC group than in the control group (p<0.05).Conclusions In wounds with exposed bone, autogenic ADSCs can promote vascularization and wound healing. Use of this cell source has multiple benefits, including convenient clinical application and lack of ethical concerns.