RT Journal Article SR Electronic T1 Early functional mismatch between breast cancer cells and their tumour microenvironment suppresses long term growth JF bioRxiv FD Cold Spring Harbor Laboratory SP 2021.06.15.448466 DO 10.1101/2021.06.15.448466 A1 Anna Perdrix Rosell A1 Oscar Maiques A1 Probir Chakravarty A1 Luigi Ombrato A1 Victoria Sanz-Moreno A1 Ilaria Malanchi YR 2021 UL http://biorxiv.org/content/early/2021/11/18/2021.06.15.448466.abstract AB Cancer cells thrive embedded in a fine-tuned cellular and extracellular environment or tumour microenvironment (TME). There is a general understanding of a co-evolution between cancer cells and their surrounding TME, pointing at a functional connection between cancer cells characteristics and the perturbations induced in their surrounding tissue. However, whether this functional connection needs to be set from the start or if aggressive cancer cells can always be dominating their microenvironment has never been formally proven with a dedicated experimental setting where malignant cells can be challenged to grow in a different TME from the one they would naturally create. Here we generated an experimental setting where we transiently perturb the secretory profile of aggressive breast cancer cells without affecting their intrinsic growth ability. This led to the initial establishment of an atypical TME. Interestingly, even if initially tumours are formed, this atypical TME evolves to impair long term in vivo cancer growth. Using a combination of in vivo transcriptomics, protein arrays and in vitro co-cultures, we found that the atypical TME culminates in the infiltration of macrophages with STAT1high activity. These macrophages show strong anti-tumoural functions which reduce long-term tumour growth, despite lacking canonical M1 markers. Importantly, gene signatures of the mesenchymal compartment of the TME, as well as the anti-tumoural macrophages show striking prognostic power-correlating with less aggressive human breast cancers.Competing Interest StatementThe authors have declared no competing interest.