RT Journal Article
SR Electronic
T1 Aerobic glycolysis in cancer cells supplies ATP while preventing excess metabolic thermogenesis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.11.16.468557
DO 10.1101/2021.11.16.468557
A1 Nobuyuki Okahashi
A1 Tomoki Shima
A1 Yuya Kondo
A1 Chie Araki
A1 Shuma Tsuji
A1 Akane Sawai
A1 Hikaru Uehara
A1 Susumu Kohno
A1 Hiroshi Shimizu
A1 Chiaki Takahashi
A1 Fumio Matsuda
YR 2021
UL http://biorxiv.org/content/early/2021/11/19/2021.11.16.468557.abstract
AB A general feature of cancer metabolism is ATP regeneration via substrate-level phosphorylation even under normoxic conditions (aerobic glycolysis). However, it is unclear why cancer cells prefer the inefficient aerobic glycolysis over the highly efficient process of oxidative phosphorylation for ATP regeneration. Here, we show that a beneficial aspect of aerobic glycolysis is that it reduces metabolic heat generation during ATP regeneration. 13C-metabolic flux analysis of 12 cultured cancer cell lines and in silico metabolic simulation revealed that metabolic heat production during ATP regeneration via aerobic glycolysis was considerably lesser than that produced via oxidative phosphorylation. The dependency on aerobic glycolysis was partly alleviated upon culturing under low temperatures. In conclusion, thermogenesis is required for maintaining thermal homeostasis and can govern aerobic glycolysis in cancer cells.Competing Interest StatementThe authors have declared no competing interest.