RT Journal Article
SR Electronic
T1 Restoring single-molecule localizations with wavefront sensing adaptive optics for deep-tissue super-resolution imaging
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.11.18.469175
DO 10.1101/2021.11.18.469175
A1 Sanghyeon Park
A1 Yonghyeon Jo
A1 Minsu Kang
A1 Jin Hee Hong
A1 Sangyoon Ko
A1 Suhyun Kim
A1 Sangjun Park
A1 Hae-Chul Park
A1 Sang-Hee Shim
A1 Wonshik Choi
YR 2021
UL http://biorxiv.org/content/early/2021/11/20/2021.11.18.469175.abstract
AB Specimen-induced aberration has been one of the major factors limiting the imaging depth in single-molecule localization microscopy (SMLM). In this study, we measured the wavefront of intrinsic reflectance signal at the fluorescence emission wavelength to construct a time-gated reflection matrix and find complex tissue aberration without resorting to fluorescence detection. Physically correcting the identified aberration via wavefront shaping with a liquid-crystal spatial light modulator (SLM)enables super-resolution imaging even when the aberration is too severe for initiating localization processes. We demonstrate the correction of complex tissue aberration, the root-mean-square (RMS)wavefront distortion of which is more than twice the 1 rad limit presented in previous studies; this leads to the recovery of single molecules by 77 times increased localization number. We visualised dendritic spines in mouse brain tissues and early myelination processes in a whole zebrafish at up to 102 μm depth with 28–39 nm localization precision. The proposed approach can expand the application range of SMLM to thick samples that cause the loss of localization points owing to severe aberration.Competing Interest StatementThe authors have declared no competing interest.