RT Journal Article
SR Electronic
T1 Label-free imaging of collagen fibers in tissue slices using phase imaging with computational specificity
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.11.19.469223
DO 10.1101/2021.11.19.469223
A1 Masayoshi Sakakura
A1 Virgilia Macias
A1 André Kajdacsy-Balla
A1 Gabriel Popescu
YR 2021
UL http://biorxiv.org/content/early/2021/11/20/2021.11.19.469223.abstract
AB Evaluating the tissue collagen content in addition to the epithelial morphology has been proven to offer complementary information in histopathology, especially in disease stratification and patient survivability prediction. One imaging modality widely used for this purpose is second harmonic generation microscopy (SHGM), which reports on the nonlinear susceptibility associated with the collagen fibers. Another method is polarization light microscopy (PLM) combined with picrosirius-red (PSR) tissue staining. However, SHGM requires expensive equipment and provides limited throughput, while PLM and PSR staining are not part of the routine pathology workflow. Here, we advance phase imaging with computational specificity (PICS) to computationally infer the collagen distribution of unlabeled tissue, with high specificity. PICS utilizes deep learning to translate quantitative phase images (QPI) into corresponding PSR images with high accuracy and speed. Our results indicate that the distributions of collagen fiber orientation, length, and straightness reported by PICS closely match the ones from ground truth.Competing Interest StatementThe authors have declared no competing interest.