PT  - JOURNAL ARTICLE
AU  - R. Patrick Xian
AU  - Claire L. Walsh
AU  - Stijn E. Verleden
AU  - Willi L. Wagner
AU  - Alexandre Bellier
AU  - Sebastian Marussi
AU  - Maximilian Ackermann
AU  - Danny D. Jonigk
AU  - Joseph Jacob
AU  - Peter D. Lee
AU  - Paul Tafforeau
TI  - A multiscale X-ray phase-contrast tomography dataset of whole human left lung
AID  - 10.1101/2021.11.20.469361
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.11.20.469361
4099  - http://biorxiv.org/content/early/2021/11/20/2021.11.20.469361.short
4100  - http://biorxiv.org/content/early/2021/11/20/2021.11.20.469361.full
AB  - Technological advancements in X-ray imaging using bright and coherent synchrotron sources now allows to decouple sample size and resolution, while maintaining high sensitivity to the microstructure of soft, partially dehydrated tissues. The recently developed imaging technique, hierarchical phase-contrast tomography, is a comprehensive approach to address the challenge of organ-scale (up to tens of centimeters) soft tissue imaging with resolution and sensitivity down to the cellular level. Using this technique, we imaged ex vivo an entire human left lung at an isotropic voxel size of 25.08 μm along with local zooms down to 6.05 - 6.5 μm and 2.45 - 2.5 μm in voxel size. The high tissue contrast offered by the fourth-generation synchrotron source at the European Synchrotron Radiation Facility reveals complex multiscale anatomical constitution of the human lung from the macroscopic (centimeter) down to the microscopic (micrometer) scale. The dataset provides complete organ-scale 3D information of the secondary pulmonary lobules and delineates the microstructure of lung nodules with unprecedented detail.Competing Interest StatementThe authors have declared no competing interest.