PT  - JOURNAL ARTICLE
AU  - Sachin G. Chavan
AU  - Remko A. Duursma
AU  - Michael Tausz
AU  - Oula Ghannoum
TI  - Heat stress prevented the biomass and yield stimulation caused by elevated CO<sub>2</sub> in two well-watered wheat cultivars
AID  - 10.1101/2021.11.21.469459
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.11.21.469459
4099  - http://biorxiv.org/content/early/2021/11/22/2021.11.21.469459.short
4100  - http://biorxiv.org/content/early/2021/11/22/2021.11.21.469459.full
AB  - To investigate the interactive effects of elevated CO2 and heat stress (HS), we grew two contrasting wheat cultivars, early-maturing Scout and high-tillering Yitpi, under non-limiting water and nutrients at ambient (aCO2, 450 ppm) or elevated (eCO2, 650 ppm) CO2 and 22°C in the glasshouse. Plants were exposed to two 3-day HS cycles at the vegetative (38.1°C) and/or flowering (33.5°C) stage.At aCO2, both wheat cultivars showed similar responses of photosynthesis and mesophyll conductance to temperature and produced similar grain yield. Relative to aCO2, eCO2 enhanced photosynthesis rate and reduced stomatal conductance and maximal carboxylation rate (Vcmax). During HS, high temperature stimulated photosynthesis at eCO2 in both cultivars, while eCO2 stimulated photosynthesis in Scout. Electron transport rate (Jmax) was unaffected by any treatment. eCO2 equally enhanced biomass and grain yield of both cultivars in control, but not HS, plants. HS reduced biomass and yield of Scout at eCO2. Yitpi, the cultivar with higher grain nitrogen, underwent a trade-off between grain yield and nitrogen. In conclusion, eCO2 improved photosynthesis of control and HS wheat, and improved biomass and grain yield of control plants only. Under well-watered conditions, HS was not detrimental to photosynthesis or growth but precluded a yield response to eCO2.Key message High temperatures increased photosynthetic rates only at eCO2 and photosynthesis was upregulated after recovery from heat stress at eCO2 in Scout suggesting that eCO2 increased optimum temperature of photosynthesis.Competing Interest StatementThe authors have declared no competing interest.