PT - JOURNAL ARTICLE AU - David Pastor-Escuredo AU - Benoît Lombardot AU - Thierry Savy AU - Adeline Boyreau AU - Jose M. Goicolea AU - Andrés Santos AU - Paul Bourgine AU - Juan C. del Álamo AU - Nadine Peyriéras AU - María J. Ledesma-Carbayo TI - Kinematic analysis of cell lineage reveals coherent and robust mechanical deformation patterns in zebrafish gastrulation AID - 10.1101/054353 DP - 2016 Jan 01 TA - bioRxiv PG - 054353 4099 - http://biorxiv.org/content/early/2016/10/19/054353.short 4100 - http://biorxiv.org/content/early/2016/10/19/054353.full AB - Digital cell lineages reconstructed from 3D+time imaging data provide unique information to unveil mechanical cues and their role in morphogenetic processes. Our methodology based on a kinematic analysis of cell lineage data reveals deformation patterns and quantitative morphogenetic landmarks for a new type of developmental table. The characteristic spatial and temporal length scales of mechanical deformation patterns derived from a continuous approximation of cell displacements indicate a compressible fluid-like behavior of zebrafish gastrulating tissues. The instantaneous deformation rate at the mesoscopic level of the cell’s neighborhood is spatially and temporally heterogeneous. The robustness of mechanical patterns results from their cumulative history along cell trajectories. Unsupervised classification of mechanical descriptor profiles was used to assess the homogeneity of biomechanical cues in cell populations. Further clustering of cell trajectories according to their cumulative mesoscopic biomechanical history during gastrulation revealed ordered and coherent spatiotemporal patterns comparable to that of the embryonic fate map.