RT Journal Article
SR Electronic
T1 Learning the rules of cell competition without prior scientific knowledge
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.11.24.469554
DO 10.1101/2021.11.24.469554
A1 Soelistyo, Christopher J.
A1 Vallardi, Giulia
A1 Charras, Guillaume
A1 Lowe, Alan R.
YR 2021
UL http://biorxiv.org/content/early/2021/11/25/2021.11.24.469554.abstract
AB Deep learning is now a powerful tool in microscopy data analysis, and is routinely used for image processing applications such as segmentation and denoising. However, it has rarely been used to directly learn mechanistic models of a biological system, owing to the complexity of the internal representations. Here, we develop an end-to-end machine learning model capable of learning the rules of a complex biological phenomenon, cell competition, directly from a large corpus of time-lapse microscopy data. Cell competition is a quality control mechanism that eliminates unfit cells from a tissue and during which cell fate is thought to be determined by the local cellular neighborhood over time. To investigate this, we developed a new approach (τ-VAE) by coupling a variational autoencoder to a temporal convolution network to predict the fate of each cell in an epithelium. Using the τ-VAE’s latent representation of the local tissue organization and the flow of information in the network, we decode the physical parameters responsible for correct prediction of fate in cell competition. Remarkably, the model autonomously learns that cell density is the single most important factor in predicting cell fate – a conclusion that has taken over a decade of traditional experimental research to reach. Finally, to test the learned internal representation, we challenge the network with experiments performed in the presence of drugs that block signalling pathways involved in competition. We present a novel discriminator network that, using the predictions of the τ-VAE, can identify conditions which deviate from the normal behaviour, paving the way for automated, mechanism-aware drug screening.Competing Interest StatementThe authors have declared no competing interest.