PT  - JOURNAL ARTICLE
AU  - Sumit Tarafder
AU  - Mazharul Islam
AU  - Swakkhar Shatabda
AU  - Atif Rahman
TI  - Figbird: A probabilistic method for filling gaps in genome assemblies
AID  - 10.1101/2021.11.24.469861
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.11.24.469861
4099  - http://biorxiv.org/content/early/2021/11/25/2021.11.24.469861.short
4100  - http://biorxiv.org/content/early/2021/11/25/2021.11.24.469861.full
AB  - Motivation Advances in sequencing technologies have led to sequencing of genomes of a multitude of organisms. However, draft genomes of many of these organisms contain a large number of gaps due to repeats in genomes, low sequencing coverage and limitations in sequencing technologies. Although there exist several tools for filling gaps, many of these do not utilize all information relevant to gap filling.Results Here, we present a probabilistic method for filling gaps in draft genome assemblies using second generation reads based on a generative model for sequencing that takes into account information on insert sizes and sequencing errors. Our method is based on the expectation-maximization (EM) algorithm unlike the graph based methods adopted in the literature. Experiments on real biological datasets show that this novel approach can fill up large portions of gaps with small number of errors and misassemblies compared to other state of the art gap filling tools.Availability and Implementation The method is implemented using C++ in a software named “Filling Gaps by Iterative Read Distribution (Figbird)”, which is available at: https://github.com/SumitTarafder/Figbird.Contact atif{at}cse.buet.ac.bdSupplementary information Supplementary data are available at Bioinformatics online.Competing Interest StatementThe authors have declared no competing interest.