TY - JOUR T1 - Simultaneous pathway engagement of translation in astrocytes, circadian rhythm in GABAergic neurons and cytoskeleton in glutamatergic neurons precede electroencephalographic changes in neurodegenerative prion disease JF - bioRxiv DO - 10.1101/2021.11.26.470101 SP - 2021.11.26.470101 AU - Lech Kaczmarczyk AU - Melvin Schleif AU - Lars Dittrich AU - Rhiannan Williams AU - MaruĊĦa Koderman AU - Vikas Bansal AU - Ashish Rajput AU - Theresa Schulte AU - Maria Jonson AU - Clemens Krost AU - Fabio Testaquadra AU - Stefan Bonn AU - Walker S. Jackson Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/11/27/2021.11.26.470101.abstract N2 - Selective vulnerability is an enigmatic feature of neurodegenerative diseases (NDs), whereby a widely expressed protein causes lesions in specific brain regions and cell types. This selectivity may arise from cells possessing varying capacities to regain proteostasis when stressed by cytotoxic protein conformers. Using the RiboTag method in mice, translational responses of five neural subtypes to acquired prion disease (PrD) were measured. Pre-onset and disease onset timepoints were chosen based on longitudinal electroencephalography (EEG) that revealed a gradual increase in theta power between 10- and 18-weeks after prion injection, resembling a clinical feature of human PrD. At disease onset, marked by significantly increased theta power and histopathological lesions, mice had pronounced translatome changes in all five cell types despite having a normal outward appearance. Remarkably, at a pre-onset stage, prior to EEG and neuropathological changes, we found that 1) translatomes of astrocytes indicated a sharply reduced synthesis of ribosomal and mitochondrial components, 2) excitatory neurons showed increased expression of cytoskeletal genes, and 3) inhibitory neurons revealed reduced expression of circadian rhythm network genes. Further assessment for the role of circadian rhythms using a jet lag paradigm modestly exacerbated disease. These data demonstrate that early translatome responses to neurodegeneration emerge prior to other signs of disease and are unique to different cell types. Therapeutic strategies may need to target multiple pathways, each in specific populations of cells, early in the disease process.Competing Interest StatementThe authors have declared no competing interest. ER -