RT Journal Article
SR Electronic
T1 Endocytic Trafficking Promotes Vacuolar Enlargements for Fast Cell Expansion Rates in Plants
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.11.29.470358
DO 10.1101/2021.11.29.470358
A1 Kai Dünser
A1 Maria Schöller
A1 Christian Löfke
A1 Nannan Xiao
A1 Barbora Pařízková
A1 Stanislav Melnik
A1 Eva Stöger
A1 Ondřej Novák
A1 Jürgen Kleine-Vehn
YR 2021
UL http://biorxiv.org/content/early/2021/11/30/2021.11.29.470358.abstract
AB The vacuole has a space-filling function, allowing a particularly rapid plant cell expansion with very little increase in cytosolic content (Löfke et al., 2015; Scheuring et al., 2016; Dünser et al., 2019). Despite its importance for cell size determination in plants, very little is known about the mechanisms that define vacuolar size. Here we show that the cellular and vacuolar size expansions are coordinated. By developing a pharmacological tool, we enabled the investigation of membrane delivery to the vacuole during cellular expansion. Counterintuitively, our data reveal that endocytic trafficking from the plasma membrane to the vacuole is enhanced in the course of rapid root cell expansion. While this “compromise” mechanism may theoretically at first decelerate cell surface enlargements, it fuels vacuolar expansion and, thereby, ensures the coordinated augmentation of vacuolar occupancy in dynamically expanding plant cells.Competing Interest StatementThe authors have declared no competing interest.