TY - JOUR T1 - Endocytic Trafficking Promotes Vacuolar Enlargements for Fast Cell Expansion Rates in Plants JF - bioRxiv DO - 10.1101/2021.11.29.470358 SP - 2021.11.29.470358 AU - Kai Dünser AU - Maria Schöller AU - Christian Löfke AU - Nannan Xiao AU - Barbora Pařízková AU - Stanislav Melnik AU - Eva Stöger AU - Ondřej Novák AU - Jürgen Kleine-Vehn Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/11/30/2021.11.29.470358.abstract N2 - The vacuole has a space-filling function, allowing a particularly rapid plant cell expansion with very little increase in cytosolic content (Löfke et al., 2015; Scheuring et al., 2016; Dünser et al., 2019). Despite its importance for cell size determination in plants, very little is known about the mechanisms that define vacuolar size. Here we show that the cellular and vacuolar size expansions are coordinated. By developing a pharmacological tool, we enabled the investigation of membrane delivery to the vacuole during cellular expansion. Counterintuitively, our data reveal that endocytic trafficking from the plasma membrane to the vacuole is enhanced in the course of rapid root cell expansion. While this “compromise” mechanism may theoretically at first decelerate cell surface enlargements, it fuels vacuolar expansion and, thereby, ensures the coordinated augmentation of vacuolar occupancy in dynamically expanding plant cells.Competing Interest StatementThe authors have declared no competing interest. ER -