PT  - JOURNAL ARTICLE
AU  - Rajdeep Chakraborty
AU  - Amara Jabeen
AU  - Honghua Hu
AU  - Charbel Darido
AU  - Karen Vickery
AU  - Shoba Ranganathan
TI  - TRPA1: A Potential Prognostic Indicator for Oral Squamous Cell Carcinoma
AID  - 10.1101/2021.11.29.470496
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.11.29.470496
4099  - http://biorxiv.org/content/early/2021/11/30/2021.11.29.470496.short
4100  - http://biorxiv.org/content/early/2021/11/30/2021.11.29.470496.full
AB  - Introduction Transient receptors are related to oral cancer pain. Previously capsaicin (TRPV1 agonist) was shown to induce cell death in oral cancer cells. We hypothesised that these receptors are present in oral cancer.Method We examined the presence of cannabinoid receptors (CB1 and CB2) and targets (TRPV1, TRPA1, CaV 3.1, CaV 3.2, CaV 3.3) via quantitative polymerase chain reaction (qPCR) in oral cancer cells SCC4, SCC9, SCC25, Cal27, and normal oral cell line OKF6.Result Cannabinoid receptors are absent in all the cell lines, while TRPA1 is only present in normal cells, but absent in all the oral cancer cell lines. Voltage-gated calcium channels are present in all the cell lines.Conclusion and Future Aspects TRPA1 could be the possible future prognostic indicator of oral squamous cell carcinoma. Future functionality assays could use precancerous cell lines to follow the loss of TRPA1.Competing Interest StatementThe authors have declared no competing interest.