RT Journal Article
SR Electronic
T1 TRPA1: A Potential Prognostic Indicator for Oral Squamous Cell Carcinoma
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.11.29.470496
DO 10.1101/2021.11.29.470496
A1 Chakraborty, Rajdeep
A1 Jabeen, Amara
A1 Hu, Honghua
A1 Darido, Charbel
A1 Vickery, Karen
A1 Ranganathan, Shoba
YR 2021
UL http://biorxiv.org/content/early/2021/11/30/2021.11.29.470496.abstract
AB Introduction Transient receptors are related to oral cancer pain. Previously capsaicin (TRPV1 agonist) was shown to induce cell death in oral cancer cells. We hypothesised that these receptors are present in oral cancer.Method We examined the presence of cannabinoid receptors (CB1 and CB2) and targets (TRPV1, TRPA1, CaV 3.1, CaV 3.2, CaV 3.3) via quantitative polymerase chain reaction (qPCR) in oral cancer cells SCC4, SCC9, SCC25, Cal27, and normal oral cell line OKF6.Result Cannabinoid receptors are absent in all the cell lines, while TRPA1 is only present in normal cells, but absent in all the oral cancer cell lines. Voltage-gated calcium channels are present in all the cell lines.Conclusion and Future Aspects TRPA1 could be the possible future prognostic indicator of oral squamous cell carcinoma. Future functionality assays could use precancerous cell lines to follow the loss of TRPA1.Competing Interest StatementThe authors have declared no competing interest.