PT - JOURNAL ARTICLE AU - McMahon, Lloyd P. AU - Digilio, Laura AU - Duston, Alois AU - Yap, Chan Choo AU - Winckler, Bettina TI - KymoMerge: a new tool for analysis of multichannel kymographs AID - 10.1101/2021.11.29.470387 DP - 2021 Jan 01 TA - bioRxiv PG - 2021.11.29.470387 4099 - http://biorxiv.org/content/early/2021/11/30/2021.11.29.470387.short 4100 - http://biorxiv.org/content/early/2021/11/30/2021.11.29.470387.full AB - Kymograph analysis is commonly used by researchers to study dynamic processes in cells. Current tools widely available only allow for analysis of one channel kymographs. Here we provide a python-coded, open source program to use as a plug-in for ImageJ which creates one kymograph from the kymographs of two separate channels of one time-lapse movie. This add-in, which we call KymoMerge, therefore allows for analysis of only the co-located tracks in multi-channel movies.Competing Interest StatementThe authors have declared no competing interest.