RT Journal Article SR Electronic T1 KymoMerge: a new tool for analysis of multichannel kymographs JF bioRxiv FD Cold Spring Harbor Laboratory SP 2021.11.29.470387 DO 10.1101/2021.11.29.470387 A1 McMahon, Lloyd P. A1 Digilio, Laura A1 Duston, Alois A1 Yap, Chan Choo A1 Winckler, Bettina YR 2021 UL http://biorxiv.org/content/early/2021/11/30/2021.11.29.470387.abstract AB Kymograph analysis is commonly used by researchers to study dynamic processes in cells. Current tools widely available only allow for analysis of one channel kymographs. Here we provide a python-coded, open source program to use as a plug-in for ImageJ which creates one kymograph from the kymographs of two separate channels of one time-lapse movie. This add-in, which we call KymoMerge, therefore allows for analysis of only the co-located tracks in multi-channel movies.Competing Interest StatementThe authors have declared no competing interest.