PT  - JOURNAL ARTICLE
AU  - Shi, Peiguo
AU  - Murphy, Michael R.
AU  - Aparicio, Alexis O.
AU  - Kesner, Jordan S.
AU  - Fang, Zhou
AU  - Chen, Ziheng
AU  - Trehan, Aditi
AU  - Wu, Xuebing
TI  - RNA-guided cell targeting with CRISPR/RfxCas13d collateral activity in human cells
AID  - 10.1101/2021.11.30.470032
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.11.30.470032
4099  - http://biorxiv.org/content/early/2021/11/30/2021.11.30.470032.short
4100  - http://biorxiv.org/content/early/2021/11/30/2021.11.30.470032.full
AB  - While single-cell sequencing has allowed rapid identification of novel cell types or states and associated RNA markers, functional studies remain challenging due to the lack of tools that are able to target specific cells based on these markers. Here we show that targeting a single marker RNA with CRISPR/RfxCas13d led to collateral transcriptome destruction in human cells, which can be harnessed to inhibit cell proliferation or to suppress cell state transition.Competing Interest StatementThe authors have declared no competing interest.