RT Journal Article
SR Electronic
T1 RNA-guided cell targeting with CRISPR/RfxCas13d collateral activity in human cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.11.30.470032
DO 10.1101/2021.11.30.470032
A1 Shi, Peiguo
A1 Murphy, Michael R.
A1 Aparicio, Alexis O.
A1 Kesner, Jordan S.
A1 Fang, Zhou
A1 Chen, Ziheng
A1 Trehan, Aditi
A1 Wu, Xuebing
YR 2021
UL http://biorxiv.org/content/early/2021/11/30/2021.11.30.470032.abstract
AB While single-cell sequencing has allowed rapid identification of novel cell types or states and associated RNA markers, functional studies remain challenging due to the lack of tools that are able to target specific cells based on these markers. Here we show that targeting a single marker RNA with CRISPR/RfxCas13d led to collateral transcriptome destruction in human cells, which can be harnessed to inhibit cell proliferation or to suppress cell state transition.Competing Interest StatementThe authors have declared no competing interest.