TY - JOUR T1 - A temporal sequence of thalamic activity unfolds at transitions in behavioral arousal state JF - bioRxiv DO - 10.1101/2021.12.01.470627 SP - 2021.12.01.470627 AU - Beverly Setzer AU - Nina E. Fultz AU - Daniel E. P. Gomez AU - Stephanie D. Williams AU - Giorgio Bonmassar AU - Jonathan R. Polimeni AU - Laura D. Lewis Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/12/02/2021.12.01.470627.abstract N2 - The moment of awakening from sleep reflects a profound transformation in neural activity and behavior. The thalamus is a key controller of arousal state, but whether its diverse nuclei exhibit coordinated or distinct activity at transitions in behavioral arousal state is not known. Using fast fMRI at ultra-high field (7 Tesla), we measured sub-second activity across thalamocortical networks and within nine thalamic nuclei to delineate these dynamics during spontaneous transitions in behavioral arousal state. We discovered a stereotyped sequence of activity across thalamic nuclei that preceded behavioral arousal after a period of inactivity, followed by widespread cortical deactivation. These thalamic dynamics were linked to whether participants remained awake or fell back asleep, with unified thalamic activation reflecting subsequent maintenance of awake behavior. These results provide an outline of the complex interactions across thalamocortical circuits that orchestrate arousal state transitions, and additionally, demonstrate that fast fMRI can resolve sub-second subcortical dynamics in the human brain.Competing Interest StatementThe authors have declared no competing interest. ER -