RT Journal Article
SR Electronic
T1 A Non-Canonical Function of Arabidopsis ERECTA Proteins in Gibberellin Signaling
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.12.02.470991
DO 10.1101/2021.12.02.470991
A1 Elzbieta Sarnowska
A1 Szymon Kubala
A1 Pawel Cwiek
A1 Sebastian Sacharowski
A1 Paulina Oksinska
A1 Jaroslaw Steciuk
A1 Magdalena Zaborowska
A1 Jakub M. Szurmak
A1 Roman Dubianski
A1 Anna Maassen
A1 Malgorzata Stachowiak
A1 Bruno Huettel
A1 Monika Ciesla
A1 Klaudia Kogut
A1 Anna T. Rolicka
A1 Saleh Alseekh
A1 Ernest Bucior
A1 Rainer Franzen
A1 Anna Klepacz
A1 Malgorzata A. Domagalska
A1 Samija Amar
A1 Janusz A. Siedlecki
A1 Alisdair R. Fernie
A1 Seth J. Davis
A1 Tomasz J. Sarnowski
YR 2021
UL http://biorxiv.org/content/early/2021/12/03/2021.12.02.470991.abstract
AB The Arabidopsis ERECTA family (ERf) of leucine-rich repeat receptor-like kinases (LRR-RLKs), comprising ERECTA (ER), ERECTA-LIKE 1 (ERL1) and ERECTA-LIKE 2 (ERL2), control epidermal patterning, inflorescence architecture, stomata development, and hormonal signaling. Here we show that the er/erl1/erl2 triple mutant exhibits impaired gibberellin (GA) biosynthesis and perception alongside broad transcriptional changes. ERf proteins interact in the nucleus, via kinase domains, with the SWI3B subunit of the SWI/SNF chromatin remodeling complex (CRCs). The er/erl1/erl2 triple mutant exhibits reduced SWI3B protein level and affected nucleosomal chromatin structure. The ER kinase phosphorylates SWI3B in vitro, and the inactivation of all ERf proteins leads to the decreased phosphorylation of SWI3B protein in vivo. Correlation between DELLA overaccumulation and SWI3B proteasomal degradation together with the physical interaction of SWI3B with DELLA proteins explain the lack of RGA accumulation in the GA- and SWI3B-deficient erf mutant plants. Co-localization of ER and SWI3B on GID1 (GIBBERELLIN INSENSITIVE DWARF 1) DELLA target gene promoter regions and abolished SWI3B binding to GID1 promoters in er/erl1/erl2 plants supports the conclusion that ERf-SWI/SNF CRC interaction is important for transcriptional control of GA receptors. Thus, the involvement of ERf proteins in transcriptional control of gene expression, and observed similar features for human HER2 (Epidermal Growth Family Receptor-member), indicate an exciting target for further studies of evolutionarily conserved non-canonical functions of eukaryotic membrane receptors.ONE SENTENCE SUMMARY ERECTA leucine-rich receptor-like kinase and SWI3B subunit of SWI/SNF chromatin remodeling complex cooperate in direct transcriptional control of GID1 genes in Arabidopsis.Competing Interest StatementThe authors have declared no competing interest.ERfERECTA familyERERECTAERL1ERECTA-LIKE 1ERL2ERECT-LIKE 2LRR-RLKsleucine-rich repeat receptor-like kinasesCRCchromatin remodeling complexSWI/SNFSwitch/Sucrose NonfermentingGID1GIBBERELLIN INSENSITIVE DWARF 1GAgibberellinPACPaclobutrazolqRT-PCRquantitative real-time PCRBFABrefeldin ANLSnuclear localization signalKDERthe kinase domain of ERECTATSStranscription start siteEGFRepidermal growth factor receptor