RT Journal Article
SR Electronic
T1 CV-containing vesicle budding from chloroplast inner envelope membrane is mediated by clathrin but inhibited by GAPC
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.12.02.470903
DO 10.1101/2021.12.02.470903
A1 Ting Pan
A1 Yangxuan Liu
A1 Chengcheng Ling
A1 Yuying Tang
A1 Wei Tang
A1 Yongsheng Liu
A1 Liang Guo
A1 Chanhong Kim
A1 Jun Fang
A1 Honghui Lin
A1 Eduardo Blumwald
A1 Songhu Wang
YR 2021
UL http://biorxiv.org/content/early/2021/12/03/2021.12.02.470903.abstract
AB Clathrin-mediated vesicular formation and trafficking are highly conserved in eukaryotic cells and are responsible for molecular cargo transport and signal transduction among organelles. It remains largely unknown whether clathrin-coated vesicles can be generated from chloroplasts. CHLOROPLAST VESICULATION (CV)-containing vesicles (CVVs) generate from chloroplasts and mediate chloroplast degradation under abiotic stress. In this study, we showed that CV interacted with the clathrin heavy chain (CHC) and induced vesicle budding from the chloroplast inner envelope membrane. Defects on CHC2 and the dynamin-encoding DRP1A gene affected CVV budding and releasing from chloroplast. CHC2 is also required for CV-induced chloroplast degradation and hypersensitivity to water stress. Moreover, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (GAPC) interacts with CV and impairs the CV-CHC2 interaction. GAPC1 overexpression inhibited CV-mediated chloroplast degradation and hypersensitivity to water stress. CV silencing alleviated the hypersensitivity of gapc1gapc2 plant to water stress. Together, our work revealed a pathway of clathrin-assisted CVV budding from the chloroplast inner envelope membrane, which mediated the stress-induced chloroplast degradation and stress response.