PT  - JOURNAL ARTICLE
AU  - Chamteut Oh
AU  - Ratul Chowdhury
AU  - Laxmicharan Samineni
AU  - Joanna L Shisler
AU  - Manish Kumar
AU  - Thanh H. Nguyen
TI  - Inactivation mechanism and efficacy of grape seed extract for Human Norovirus surrogate
AID  - 10.1101/2021.12.03.471102
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.12.03.471102
4099  - http://biorxiv.org/content/early/2021/12/04/2021.12.03.471102.short
4100  - http://biorxiv.org/content/early/2021/12/04/2021.12.03.471102.full
AB  - Proper disinfection of harvested food and water is critical to minimize infectious disease. Grape seed extract (GSE), a commonly used health supplement, is a mixture of plant-derived polyphenols. Polyphenols possess anti-microbial and -fungal properties, but anti-viral effects are not well-known. Here we show that GSE outperformed chemical disinfectants (e.g., free chlorine and peracetic acids) in inactivating Tulane virus, a human norovirus surrogate. GSE induced virus aggregation, an event that correlated with a decrease in virus titers. This aggregation and disinfection was not reversible. Molecular docking simulations indicate that polyphenols potentially formed hydrogen bonds and strong hydrophobic interactions with specific residues in viral capsid proteins. Together, these data suggest that polyphenols physically associate with viral capsid proteins to aggregate viruses as a means to inhibit virus entry into the host cell. Plant-based polyphenols like GSE are an attractive alternative to chemical disinfectants to remove infectious viruses from water or food.Importance Human noroviruses are major food- and water-borne pathogens, causing approximately 20% of all cases of acute gastroenteritis cases in developing and developed countries. Proper sanitation or disinfection are critical strategies to minimize human norovirus-caused disease until a reliable vaccine is created. Grape seed extract (GSE) is a mixture of plant-derived polyphenols that is used as a health supplement. Polyphenols are known for antimicrobial, antifungal, and antibiofilm activities, but antiviral effects are not well-known. In studies here, plant-derived polyphenols outperformed chemical disinfectants (e.g., free chlorine and peracetic acids) in inactivating Tulane virus, a human norovirus surrogate. Based on data from additional molecular assays and molecular docking simulations, the current model is that the polyphenols in GSE bind to the Tulane virus capsid, an event that triggers virion aggregation. It is thought that this aggregation prevents Tulane virus from entering host cells.Competing Interest StatementThe authors have declared no competing interest.