RT Journal Article
SR Electronic
T1 LKB1 Is Physiologically Required for Sleep from Drosophila to the Mouse
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.12.13.472078
DO 10.1101/2021.12.13.472078
A1 Ziyi Liu
A1 Lifen Jiang
A1 Chaoyi Li
A1 Chengang Li
A1 Jingqun Yang
A1 Dan Wang
A1 Renbo Mao
A1 Yi Rao
YR 2021
UL http://biorxiv.org/content/early/2021/12/14/2021.12.13.472078.abstract
AB LKB1 is known as a master kinase for 14 kinases related to the adenosine monophosphate (AMP)-activated protein kinase (AMPK). Two of them (SIK3 and AMPKα) have previously been implicated in sleep regulation. We generated loss-of-function (LOF) mutants for Lkb1 in both Drosophila and mice. Sleep was reduced in Lkb1-mutant flies and in flies with neuronal deletion of Lkb1. Sleep was reduced in mice after virally mediated reduction of Lkb1 in the brain. Electroencephalography (EEG) analysis showed that non-rapid eye movement (NREM) sleep and sleep need were both reduced in Lkb1-mutant mice. These results indicate that LKB1 plays a physiological role in sleep regulation conserved from flies to mice.Competing Interest StatementThe authors have declared no competing interest.