TY - JOUR T1 - Prior exposure to microcystin alters host gut resistome and is associated with dysregulated immune homeostasis in translatable mouse models JF - bioRxiv DO - 10.1101/2021.12.17.472989 SP - 2021.12.17.472989 AU - Punnag Saha AU - Dipro Bose AU - Vitalii Stebliankin AU - Trevor Cickovski AU - Ratanesh K. Seth AU - Dwayne E. Porter AU - Bryan W. Brooks AU - Kalai Mathee AU - Giri Narasimhan AU - Rita Colwell AU - Geoff I. Scott AU - Saurabh Chatterjee Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/12/18/2021.12.17.472989.abstract N2 - The increased propensity of harmful algal blooms (HABs) and exposure from HABs-cyanotoxin causes human toxicity. It has been associated with the progression of several diseases that encompass the liver, kidneys, and immune system. Recently, a strong association of cyano-HAB toxicity with the altered host gut microbiome has been shown. We tested the hypothesis that prior exposure to cyanotoxin microcystin may alter the microbiome and induce microbiome-host-resistome crosstalk. Using both wild-type and humanized mice, we show that the mice exposed to microcystin had an altered microbiome signature that harbored antimicrobial resistance genes. Host resistome phenotypes such as mefA, msrD, mel, ant6, and tet40 increased in diversity and relative abundance following microcystin exposure. Interestingly, the increased abundance of these genes was traced to resistance to common antibiotics such as tetracycline, macrolides, glycopeptide, and aminoglycosides, crucial for modern-day treatment for several diseases. Increased abundance of these genes was positively associated with increased expression of PD1, a T-cell homeostasis marker, and pleiotropic inflammatory cytokine IL-6 with a concomitant negative association with immunosurveillance markers IL7 and TLR2. Microcystin exposure also caused decreased TLR2, TLR4, and REG3G expressions, increased immunosenescence, and higher systemic levels of IL-6 in both wild-type and humanized mice. In conclusion, the results show a first-ever characterization of the host resistome of microcystin exposure and its connection to host immune status and antibiotic resistance. The results may be crucial for understanding the ability of exposed subjects to fight future bacterial infections and the progression of the debilitating disease in hospital settings.Competing Interest StatementThe authors have declared no competing interest. ER -