PT  - JOURNAL ARTICLE
AU  - Martin Piskacek
AU  - Marek Havelka
AU  - Kristina Jendruchova
AU  - Andrea Knight
AU  - Liam P. Keegan
TI  - The evolution of the 9aaTAD domain in Sp2 proteins: inactivation with valines and intron reservoirs
AID  - 10.1101/570598
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 570598
4099  - http://biorxiv.org/content/early/2019/03/09/570598.short
4100  - http://biorxiv.org/content/early/2019/03/09/570598.full
AB  - The Sp1 transcription factor has been defined as glutamine-rich activator. The Nine amino acid TransActivation Domains (9aaTAD) have been identified in numerous transcription activators. Here, we identified the conserved 9aaTAD motif in the Sp1 and in all nine members of SP family with broad natural 9aaTAD variations. We showed by the amino acid substitutions that the glutamine residues are completely dispensable for 9aaTADs function. We described the 9aaTAD domains’ origin and evolutionary history. The ancestral Sp2 gene with inactive 9aaTAD has duplicated in early chordates and created new paralogs Sp1, Sp3 and Sp4. We discovered that the accumulation of valines in the 9aaTADs correlated with the domain inactivation. The Sp2 activation domain, whose dormancy have lasted over 100 million years during chordate evolution, enabled later diversification in the Sp1-4 clade, including both repressors and activators. The new paralogs Sp1 and Sp3 activation domains have regained their original activator function by loss of valines in their 9aaTADs.