TY - JOUR T1 - A Computational Dissection of Spike protein of SARS-CoV-2 Omicron Variant JF - bioRxiv DO - 10.1101/2021.12.17.473260 SP - 2021.12.17.473260 AU - Zaira Rehman AU - Massab Umair AU - Aamer Ikram AU - Muhammad Salman AU - Syed Adnan Haider AU - Muhammad Ammar Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/12/20/2021.12.17.473260.abstract N2 - The emergence of SARS-CoV-2 omicron variant in late November, 2021 and its rapid spread to different countries, warns the health authorities to take initiative to work on containing its spread. The omicron SARS-CoV-2 variant is unusual from the other variants of concerns reported earlier as it harbors many novel mutations in its genome particularly with >30 mutations in the spike glycoprotein alone. The current study investigated the variation in binding mechanism which it carries compared to the wild type. The study also explored the interaction profile of spike-omicron with human ACE2 receptor. The structure of omicron spike glycoprotein was determined though homology modeling. The interaction analysis was performed through docking using HADDOCK followed by binding affinity calculation. Finally, the comparison of interactions were performed among spike-ACE2 complex of wild type, delta and omicron variants. The interaction analysis has revealed the involvement of highly charged and polar residues (H505, Arg498, Ser446, Arg493, and Tyr501) in the interactions. The important novel interactions in the spike-ACE2-omicron complex was observed as S494:H34, S496:D38, R498:Y41, Y501:K353, and H505:R393 and R493:D38. Moreover, the binding affinity of spike-ACE2-omicron complex (−17.6Kcal/mol) is much higher than wild type-ACE2 (−13.2Kcal/mol) and delta-ACE2 complex (−13.3Kcal/mol). These results indicate that the involvement of polar and charged residues in the interactions with ACE2 may have an impact on increased transmissibility of omicron variant.Competing Interest StatementThe authors have declared no competing interest. ER -