RT Journal Article
SR Electronic
T1 Engraftment, fate, and function of HoxB8-conditional neutrophil progenitors in the unconditioned murine host
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.12.20.473543
DO 10.1101/2021.12.20.473543
A1 Joshua T. Cohen
A1 Michael Danise
A1 Kristina D. Hinman
A1 Brittany M. Neumann
A1 Renita Johnson
A1 Zachary S. Wilson
A1 Anna Chorzalska
A1 Patrycja M. Dubielecka
A1 Craig T. Lefort
YR 2021
UL http://biorxiv.org/content/early/2021/12/21/2021.12.20.473543.abstract
AB The development and use of murine myeloid progenitor cell lines that are conditionally immortalized through expression of HoxB8 has provided a valuable tool for studies of neutrophil biology. Recent work has extended the utility of HoxB8-conditional progenitors to the in vivo setting via their transplantation into irradiated mice. Here, we describe the isolation of HoxB8-conditional progenitor cell lines that are unique in their ability to engraft in the naïve host in the absence of conditioning of the hematopoietic niche. Our results indicate that HoxB8-conditional progenitors engraft in a β1 integrin-dependent manner and transiently generate donor-derived mature neutrophils. Furthermore, we show that neutrophils derived in vivo from transplanted HoxB8-conditional progenitors are mobilized to the periphery and recruited to sites of inflammation in a manner that depends on the C-X-C chemokine receptor 2 and β2 integrins, the same mechanisms that have been described for recruitment of endogenous primary neutrophils. Together, our studies advance the understanding of HoxB8-conditional neutrophil progenitors and describe an innovative tool that, by virtue of its ability to engraft in the naïve host, will facilitate mechanistic in vivo experimentation on neutrophils.Competing Interest StatementThe authors have declared no competing interest.