PT  - JOURNAL ARTICLE
AU  - Huayta, Javier
AU  - San-Miguel, Adriana
TI  - Endogenous DAF-16 Spatiotemporal Activity Quantitatively Predicts Lifespan Extension Induced by Dietary Restriction
AID  - 10.1101/2021.12.20.473576
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.12.20.473576
4099  - http://biorxiv.org/content/early/2021/12/21/2021.12.20.473576.short
4100  - http://biorxiv.org/content/early/2021/12/21/2021.12.20.473576.full
AB  - In many organisms, dietary restriction (DR) leads to lifespan extension through the activation of cell protection and pro-longevity gene expression programs. In the nematode C. elegans, the DAF-16 transcription factor is a key aging regulator that governs the Insulin/IGF-1 signaling pathway and undergoes translocation from the cytoplasm to the nucleus of cells when animals are exposed to food limitation. In this work, we assess the endogenous activity of DAF-16 under various DR regimes by coupling CRISPR/Cas9-enabled fluorescent tagging of DAF-16 with quantitative image analysis and machine learning. Our results indicate that lifelong DAF-16 endogenous activity is a robust predictor of mean lifespan in C. elegans, and it accounts for 78% of the lifespan variability induced by DR. We found that this lifespan-extending mechanism occurs mainly in the intestine and neurons, and that DR drives DAF-16 activity in unexpected locations such as the germline and intestinal nucleoli.Competing Interest StatementThe authors have declared no competing interest.