RT Journal Article
SR Electronic
T1 Induction of flight via midbrain projections to the cuneiform nucleus
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.12.21.473683
DO 10.1101/2021.12.21.473683
A1 Tsang, Emmy
A1 Orlandini, Camilla
A1 Sureka, Rahul
A1 Crevenna, Alvaro H.
A1 Perlas, Emerald
A1 Prankerd, Izzie
A1 Masferrer, Maria E.
A1 Gross, Cornelius T.
YR 2021
UL http://biorxiv.org/content/early/2021/12/23/2021.12.21.473683.abstract
AB The dorsal periaqueductal gray is a midbrain structure implicated in the control of defensive behaviors and the processing of painful stimuli. Electrical stimulation or optogenetic activation of excitatory neurons in dorsal periaqueductal gray results in freezing or flight behavior at low or high intensity, respectively. However, the output structures that mediate these defensive behaviors remain unconfirmed. Here we carried out a targeted classification of neuron types in dorsal periaqueductal gray using multiplex in situ sequencing and then applied cell-type and projection-specific optogenetic stimulation to identify projections from dorsal periaqueductal grey to the cuneiform nucleus that promoted goal-directed flight behavior. These data confirmed that descending outputs from dorsal periaqueductal gray serve as a trigger for directed escape behavior.Competing Interest StatementThe authors have declared no competing interest.