RT Journal Article
SR Electronic
T1 <em>Rsc3K,</em> a SMV resistance gene localized on chromosome 2 in soybean cv. Kefeng No.1, interacts with virulence determinant P3
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.12.30.474612
DO 10.1101/2021.12.30.474612
A1 Tongtong Jin
A1 Jinlong Yin
A1 Song Xue
A1 Bowen Li
A1 Tingxuan Zong
A1 Yunhua Yang
A1 Hui Liu
A1 Mengzhuo Liu
A1 Kai Xu
A1 Kai Li
A1 Liqun Wang
A1 Haijian Zhi
YR 2022
UL http://biorxiv.org/content/early/2022/01/01/2021.12.30.474612.abstract
AB Soybean mosaic virus (SMV) is one of the most devastating viral pathogens in Glycine max (L.) Merr (soybean). Twenty-two SMV strains (SC1-SC22) isolated in China were identified based on their responses to ten soybean cultivars. By using the F2-derived F3 (F2:3) and recombinant inbred line (RIL) populations of resistant Soybean cultivar (cv.) Kefeng No.1 × susceptible cv. Nannong 1138-2, we localized the gene mediating resistant to SMV-SC3 strain to a 90 kb interval on chromosome 2 in Kefeng No.1. Bean pod mottle virus (BPMV)-induced gene silencing (VIGS) were used to study the gene function of candidate genes in the mapping interval and revealed that an recombinant gene, later named as Rsc3K, caused by internal deletion of a genomic DNA frag ement in Kefeng No.1, is the resistant gene to SMV-SC3. By shuffling genes between avirulent isolate SC3 and avirulent SMV isolate 1129, we found that P3 is the virulence determinant causing resistance on Kefeng No.1. We showed the interaction between Rsc3K and P3 by the yeast two-hybrid (Y2H) and bimolecular fluorescent complementation (BiFC) assays. In conclusion, this study demonstrated that Rsc3K plays a crucial role in resistance of Kefeng No.1 to SMV-SC3 by direct interaction with viral protein P3.Highlight Rsc3K resistant to SMV-SC3 by interacting with P3.Competing Interest StatementThe authors have declared no competing interest.