PT  - JOURNAL ARTICLE
AU  - Yuxuan Ma
AU  - Zhangwei Lu
AU  - Ye Shi
AU  - Zhe Li
TI  - DNA origami as a nanomedicine for targeted rheumatoid arthritis therapy through reactive oxygen species and nitric oxide scavenging
AID  - 10.1101/2021.12.30.474611
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2021.12.30.474611
4099  - http://biorxiv.org/content/early/2022/01/01/2021.12.30.474611.short
4100  - http://biorxiv.org/content/early/2022/01/01/2021.12.30.474611.full
AB  - High levels of reactive oxygen species (ROS) and nitric oxide (NO) generated by M1 macrophages induce inflammation in the development of rheumatoid arthritis (RA). The eliminating of ROS and NO therefore represents an alternative strategy for RA treatment. Because DNA molecules possess ROS- and endogenous NO-scavenging capability, herein, we develop a nanomedicine based on triangular DNA origami nanostructures for targeted RA treatment. We showed that folic acid-modified triangular DNA origami nanostructures (FA-tDONs) could reduce ROS and NO simultaneously inside proinflammatory M1 macrophages, leading to their polarization into anti-inflammatory M2 subtype. Further in vivo studies confirmed that FA-tDONs could actively target inflamed joints in collagen-induced arthritis (CIA) mice, attenuate inflammatory cytokines and alleviate disease progression. This work demonstrated that DNA origami itself could act as a potential nanomedicine for targeted RA treatment.Competing Interest StatementThe authors have declared no competing interest.