PT  - JOURNAL ARTICLE
AU  - Schmitz, Julian
AU  - Stute, Birgit
AU  - Täuber, Sarah
AU  - Kohlheyer, Dietrich
AU  - von Lieres, Eric
AU  - Grünberger, Alexander
TI  - Reliable cell retention of mammalian suspension cells in microfluidic cultivation chambers
AID  - 10.1101/2022.01.05.475060
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.01.05.475060
4099  - http://biorxiv.org/content/early/2022/01/06/2022.01.05.475060.short
4100  - http://biorxiv.org/content/early/2022/01/06/2022.01.05.475060.full
AB  - We present a new microfluidic trapping concept to retain randomly moving suspension cells inside a cultivation chamber. In comparison to previously published complex multilayer structures, we achieve cell retention by a thin PDMS barrier, which can be easily integrated into various PDMS-based cultivation devices. Cell loss during cultivation is effectively prevented while diffusive media supply is still ensured.Competing Interest StatementThe authors have declared no competing interest.