PT  - JOURNAL ARTICLE
AU  - Christopher M. Wintersinger
AU  - Dionis Minev
AU  - Anastasia Ershova
AU  - Hiroshi M. Sasaki
AU  - Gokul Gowri
AU  - Jonathan F. Berengut
AU  - F. Eduardo Corea-Dilbert
AU  - Peng Yin
AU  - William M. Shih
TI  - Multi-micron crisscross structures from combinatorially assembled DNA-origami slats
AID  - 10.1101/2022.01.06.475243
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.01.06.475243
4099  - http://biorxiv.org/content/early/2022/01/07/2022.01.06.475243.short
4100  - http://biorxiv.org/content/early/2022/01/07/2022.01.06.475243.full
AB  - Living systems achieve robust self-assembly across length scales. Meanwhile, nanofabrication strategies such as DNA origami have enabled robust self-assembly of submicron-scale shapes.However, erroneous and missing linkages restrict the number of unique origami that can be practically combined into a single supershape. We introduce crisscross polymerization of DNA-origami slats for strictly seed-dependent growth of custom multi-micron shapes with user-defined nanoscale surface patterning. Using a library of ~2000 strands that can be combinatorially assembled to yield any of ~1e48 distinct DNA origami slats, we realize five-gigadalton structures composed of >1000 uniquely addressable slats, and periodic structures incorporating >10,000 slats. Thus crisscross growth provides a generalizable route for prototyping and scalable production of devices integrating thousands of unique components that each are sophisticated and molecularly precise.One-sentence summary Crisscross polymerization of DNA-origami slats can yield micron-scale structures with uniquely addressable nanoscale features.Competing Interest StatementA patent (PCT/US2017/045013) has been filed based on this work.