RT Journal Article
SR Electronic
T1 Multi-micron crisscross structures from combinatorially assembled DNA-origami slats
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.01.06.475243
DO 10.1101/2022.01.06.475243
A1 Wintersinger, Christopher M.
A1 Minev, Dionis
A1 Ershova, Anastasia
A1 Sasaki, Hiroshi M.
A1 Gowri, Gokul
A1 Berengut, Jonathan F.
A1 Corea-Dilbert, F. Eduardo
A1 Yin, Peng
A1 Shih, William M.
YR 2022
UL http://biorxiv.org/content/early/2022/01/07/2022.01.06.475243.abstract
AB Living systems achieve robust self-assembly across length scales. Meanwhile, nanofabrication strategies such as DNA origami have enabled robust self-assembly of submicron-scale shapes.However, erroneous and missing linkages restrict the number of unique origami that can be practically combined into a single supershape. We introduce crisscross polymerization of DNA-origami slats for strictly seed-dependent growth of custom multi-micron shapes with user-defined nanoscale surface patterning. Using a library of ~2000 strands that can be combinatorially assembled to yield any of ~1e48 distinct DNA origami slats, we realize five-gigadalton structures composed of >1000 uniquely addressable slats, and periodic structures incorporating >10,000 slats. Thus crisscross growth provides a generalizable route for prototyping and scalable production of devices integrating thousands of unique components that each are sophisticated and molecularly precise.One-sentence summary Crisscross polymerization of DNA-origami slats can yield micron-scale structures with uniquely addressable nanoscale features.Competing Interest StatementA patent (PCT/US2017/045013) has been filed based on this work.