@article {Bromage2022.01.10.475615, author = {Daniel I. Bromage and Silvia Trevelin and Josef Huntington and Victoria X. Yang and Ananya Muthukumar and Sarah J. Mackie and Greta Sawyer and Xiaohong Zhang and Celio X.C. Santos and Niloufar Safinia and Ioannis Smyrnias and Mauro Giacca and Alex Ivetic and Ajay M. Shah}, title = {Nrf2 attenuates the innate immune response after experimental myocardial infarction}, elocation-id = {2022.01.10.475615}, year = {2022}, doi = {10.1101/2022.01.10.475615}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Objectives We aimed to investigate the contribution of the transcription factor nuclear factor erythroid-derived 2-like 2 (Nrf2) to the inflammatory response after experimental myocardial infarction (MI).Background There is compelling evidence implicating dysregulated inflammation in the mechanism of ventricular remodeling and heart failure (HF) after MI. The transcription factor Nrf2 (encoded by Nfe2l2) is a promising target in this context. It impedes transcriptional upregulation of pro-inflammatory cytokines and is anti-inflammatory in various murine models.Methods We subjected Nrf2-/- mice and wild type (WT) controls to permanent left coronary artery (LCA) ligation. The inflammatory response was investigated with fluorescence-activated cell sorting (FACS) analysis of peripheral blood and heart cell suspensions, together with qRT-PCR of infarcted tissue for chemokines and their receptors. To investigate whether Nrf2-mediated transcription is a dedicated function of leukocytes, we interrogated publicly available RNA-sequencing (RNA-seq) data from mouse hearts after permanent LCA ligation for Nrf2-regulated gene (NRG) expression.Results FACS analysis demonstrated a profoundly inflamed phenotype in the hearts of global Nrf2-/- mice as compared to WT mice after MI. Moreover, infarcted tissue from Nrf2-/- mice displayed higher expression of inflammatory cytokines, chemokines, and their receptors, including IL6, Ccl2, and Cxcr4. RNA-seq analysis showed upregulated NRG expression in WT mice after MI compared to untreated mice, which was significantly higher in bioinformatically isolated CCR2+ cells.Conclusions Taken together, the results suggest that Nrf2 signalling in leukocytes, and possibly CCR2+ monocyte-derived cardiac resident macrophages, may be potential targets to prevent post-MI ventricular remodeling.Competing Interest StatementMG is a member of the Scientific Advisory Boards of Trizell Holding SA, Lausanne and DINAQR AG, Zurich-London and is founder, consultant and equity holder in Purespring Therapeutics and Forcefield Therapeutics, London, which operate in the field of gene therapy and cardioprotection. AMS reports an advisory board association with Forcefield Therapeutics, London.}, URL = {https://www.biorxiv.org/content/early/2022/01/10/2022.01.10.475615}, eprint = {https://www.biorxiv.org/content/early/2022/01/10/2022.01.10.475615.full.pdf}, journal = {bioRxiv} }