PT  - JOURNAL ARTICLE
AU  - Zhang, Yizhe
AU  - Weitz, David A.
TI  - PCR-free, label-free detection of sequence-specific DNA with single-molecule sensitivity using <em>in vitro</em> N-hybrid system in microfluidic drops
AID  - 10.1101/2022.01.09.475569
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.01.09.475569
4099  - http://biorxiv.org/content/early/2022/01/11/2022.01.09.475569.short
4100  - http://biorxiv.org/content/early/2022/01/11/2022.01.09.475569.full
AB  - We propose a novel method that can detect DNA with high specificity at the single-molecule level by employing the in vitro N-hybrid strategy realized in sub-picoliter microfluidic drops. It detects target DNA based on the specific interactions of the target-encoded proteins with their partner molecules, and achieves single-molecule sensitivity via signal-transduction and signal-amplification during gene-expression processes in a sub-picoliter droplet, therefore effectively avoiding complicated procedures in labeling-based methods or biases and artifacts in PCR-based methods.Competing Interest StatementThe authors have declared no competing interest.