PT  - JOURNAL ARTICLE
AU  - Artis Linars
AU  - Ivars Silamikelis
AU  - Dita Gudra
AU  - Ance Roga
AU  - Davids Fridmanis
TI  - OverFlap PCR – a reliable approach for generation of plasmid DNA libraries containing random sequences without template bias
AID  - 10.1101/2022.01.11.475799
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.01.11.475799
4099  - http://biorxiv.org/content/early/2022/01/11/2022.01.11.475799.short
4100  - http://biorxiv.org/content/early/2022/01/11/2022.01.11.475799.full
AB  - Over the decades the improvement of naturally occurring proteins and creation of novel ones has been the primary goal for many practical biotechnology researchers and it is widely recognized that randomization of protein sequences coupled to various effect screening methodologies is one of the most powerful techniques for fast, efficient and purposeful approach for acquisition of desired improvements. Over the years considerable advancements have been made in this field, however development of PCR based or template guided methodologies has been hampered by the resulting template sequence bias. In this article we present novel whole plasmid amplification based approach, which we named OverFlap PCR, for randomization of virtually any region of the plasmid DNA, without introduction of mentioned bias.Competing Interest StatementThe authors have declared no competing interest.