PT - JOURNAL ARTICLE AU - Ling Liu AU - Matthew T. Buckley AU - Jaime M. Reyes AU - Soochi Kim AU - Lei Tian AU - Mingqiang Wang AU - Alexander Lieu AU - Michelle Mao AU - Cristina Mateo-Rodriguez AU - Heather Ishak AU - Mira Jeong AU - Joseph C. Wu AU - Margaret A. Goodell AU - Anne Brunet AU - Thomas A. Rando TI - Exercise reprograms the inflammatory landscape of multiple stem cell compartments during mammalian aging AID - 10.1101/2022.01.12.475145 DP - 2022 Jan 01 TA - bioRxiv PG - 2022.01.12.475145 4099 - http://biorxiv.org/content/early/2022/01/12/2022.01.12.475145.short 4100 - http://biorxiv.org/content/early/2022/01/12/2022.01.12.475145.full AB - Exercise has the ability to rejuvenate stem cells and improve tissue homeostasis and regeneration in aging animals. However, the cellular and molecular changes elicited by exercise have not been systematically studied across a broad range of cell types in stem cell compartments. To gain better insight into the mechanisms by which exercise affects niche and stem cell function, we subjected young and old mice to aerobic exercise and generated a single cell transcriptomic atlas of muscle, neural and hematopoietic stem cells with their niche cells and progeny. Complementarily, we also performed whole transcriptome analysis of single myofibers from these animals. We identified common and unique pathways that are compromised across these tissues and cell types in aged animals. We found that exercise has a rejuvenating effect on subsets of stem cells, and a profound impact in the composition and transcriptomic landscape of both circulating and tissue resident immune cells. Exercise ameliorated the upregulation of a number of inflammatory pathways as well as restored aspects of cell-cell communication within these stem cell compartments. Our study provides a comprehensive view of the coordinated responses of multiple aged stem cells and niche cells to exercise at the transcriptomic level.Competing Interest StatementThe authors have declared no competing interest.