PT  - JOURNAL ARTICLE
AU  - Salehi, Arjang
AU  - Salari, Sirus
AU  - Daglian, Jennifer
AU  - Chen, Kevin
AU  - Baram, Tallie Z.
AU  - Obenaus, Andre
TI  - Vascular topology is acutely impacted by experimental febrile status epilepticus
AID  - 10.1101/2022.01.10.475698
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.01.10.475698
4099  - http://biorxiv.org/content/early/2022/01/12/2022.01.10.475698.short
4100  - http://biorxiv.org/content/early/2022/01/12/2022.01.10.475698.full
AB  - Febrile status epilepticus (FSE) is an important risk factor for temporal lobe epilepsy and early identification is vital. In a rat model of FSE, we identified an acute novel MRI signal in the basolateral amygdala (BLA) at 2 hours post FSE that predicted epilepsy in adulthood. This signal remains incompletely understood and hypothesized that it might derive from changes to vascular topology. Experimental FSE was induced in rat pups and compared to normothermic littermate controls. We examined cerebral vascular topology at 2 hours, using a novel vessel painting and analysis protocol. Blood vessel density of the cortical vasculature was significantly reduced in FSE rats, and this effect was lateralized, as reported for the MRI signal. The middle cerebral artery (MCA) exhibited abnormal topology in FSE pups but not in controls. In the BLA, significant vessel junction reductions and decreased vessel diameter were observed, together with a strong trend for reduced vessel length. In summary, FSE results in acute vascular topological changes in the cortex and BLA that may underlie the acute MRI signal that predicts progression to future epilepsy. The altered vasculature may be amenable to intervention treatments to potentially reduce the probability of progression to epilepsy following FSE.Competing Interest StatementThe authors have declared no competing interest.