PT  - JOURNAL ARTICLE
AU  - Hemalatha Bhagavan
AU  - Sujana Prabhu
AU  - Niraimathi Govindasamy
AU  - Yashoda Ghanekar
TI  - Regenerating aggregates of hydra display unique cytoskeletal organisation that is absent in a regeneration-deficient strain
AID  - 10.1101/2022.01.11.466547
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.01.11.466547
4099  - http://biorxiv.org/content/early/2022/01/12/2022.01.11.466547.short
4100  - http://biorxiv.org/content/early/2022/01/12/2022.01.11.466547.full
AB  - Hydra has the unique ability to regenerate from aggregates of dissociated single cells that lack positional information. We compared two strains of hydra, a strain of hydra that was capable of regenerating from aggregates and a strain of hydra that was deficient in this type of regeneration. We observed unique actin cytoskeletal arrangements that were present in the regenerates of regeneration-competent strain but not in the regeneration-deficient strain. Concomitantly, the regeneration-deficient strain failed to organise the extracellular cytoskeleton of laminin and collagen between ectodermal and endodermal epithelial cells. These interesting preliminary observations highlight the importance of the cytoskeletal organisation in regeneration of hydra and suggest that regeneration from the aggregates of dissociated cells through de novo patterning requires correct structural organisation of cytoskeletal elements.Competing Interest StatementThe authors have declared no competing interest.RCregeneration-competentRDregeneration-deficient