PT  - JOURNAL ARTICLE
AU  - Evan F. Fullerton
AU  - Mary C. Karom
AU  - Anne Z. Murphy
TI  - Age-induced changes in Mu Opioid Receptor signaling in the midbrain Periaqueductal Gray of male and female rats
AID  - 10.1101/2022.01.13.475945
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.01.13.475945
4099  - http://biorxiv.org/content/early/2022/01/17/2022.01.13.475945.short
4100  - http://biorxiv.org/content/early/2022/01/17/2022.01.13.475945.full
AB  - The analgesic effects of opioids are attenuated in aged rats. Opioids such as morphine produce attenuated analgesia in aged rodents compared to adults; however, the neural mechanisms underlying this attenuated response are not yet known. The present study investigated the impact of advanced age and biological sex on opioid signaling in the ventrolateral periaqueductal gray (vlPAG) in the presence of chronic inflammatory pain. Assays measuring mu opioid receptor (MOR) radioligand binding, GTPγS binding, receptor phosphorylation, cAMP inhibition, and regulator of G-protein signaling (RGS) protein expression were performed on vlPAG tissue from adult (2-3mos) and aged (16-18mos), male and female rats. Persistent inflammatory pain was induced by intraplantar injection of Complete Freund’s Adjuvant (CFA). Adult males exhibited the highest MOR binding potential and the highest G-protein activation (activation efficiency ratio) in comparison to aged males and females (adult and aged). No impact of advanced age or sex on MOR phosphorylation state was observed. DAMGO-induced cAMP inhibition was highest in the vlPAG of adult males compared to aged males and females (adult and aged). vlPAG levels of RGS4 and RGS9-2, critical for terminating G-protein signaling, were assessed using RNAscope. Adult rats (both males and females) exhibited lower levels of vlPAG RGS4 and RGS9-2 mRNA expression compared to aged males and females. The observed age-related reductions in vlPAG MOR binding potential, G-protein activation efficiency, and cAMP inhibition, along with the observed age-related increases in RGS4 and RGS9-2 vlPAG expression, provide potential mechanisms whereby the response to opioids is attenuated in the aged population. These results have significant implications for pain management in this population.HighlightsAged males and females (adult and aged) exhibit reduced vlPAG μ-opioid receptor binding potential compared to adult malesAged males and females (adult and aged) exhibit reduced opioid-induced vlPAG G-protein activation compared to adult malesAged males and females (adult and aged) exhibit reduced vlPAG MOR mediated cAMP inhibition compared to adult malesAged rats (males and females) exhibit increased vlPAG mRNA expression of Regulator of G-Protein Signaling (RGS) proteins RGS4 and RGS9-2 compared to adult rats (males and females)Competing Interest StatementThe authors have declared no competing interest.