RT Journal Article SR Electronic T1 M-TUBE: a fabrication-free microfluidic device for large-volume bacterial electroporation requiring minimal assembly JF bioRxiv FD Cold Spring Harbor Laboratory SP 2022.01.14.476275 DO 10.1101/2022.01.14.476275 A1 Po-Hsun Huang A1 Sijie Chen A1 Anthony L. Shiver A1 Rebecca Neal Culver A1 Kerwyn Casey Huang A1 Cullen R. Buie YR 2022 UL http://biorxiv.org/content/early/2022/01/17/2022.01.14.476275.abstract AB Conventional cuvette-based and microfluidics-based bacterial electroporation approaches have distinct advantages, but they are typically limited to relatively small sample volumes, reducing their utility for applications requiring high throughput such as the generation of mutant libraries. Here, we present a disposable, user-friendly microfluidic electroporation device capable of processing large volume bacterial samples yet requiring minimal device fabrication and straightforward operation. We demonstrate that the proposed device can outperform conventional cuvettes in a range of situations, including across Escherichia coli strains with a range of electroporation efficiencies, and we use its large-volume bacterial electroporation capability to generate a library of transposon mutants in the anaerobic gut commensal Bifidobacterium longum.Competing Interest StatementThe authors have declared no competing interest.