PT  - JOURNAL ARTICLE
AU  - Chanwoo Lee
AU  - Dante Lepore
AU  - Mary Munson
AU  - Tae-Young Yoon
TI  - Exocyst stimulates each step of exocytic SNARE complex assembly and vesicle fusion
AID  - 10.1101/2022.01.16.476540
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.01.16.476540
4099  - http://biorxiv.org/content/early/2022/01/17/2022.01.16.476540.short
4100  - http://biorxiv.org/content/early/2022/01/17/2022.01.16.476540.full
AB  - The exocyst is a large multisubunit tethering complex essential for targeting and fusion of secretory vesicles in eukaryotic cells. Although the assembled exocyst complex is proposed to tether vesicles to the plasma membrane and activate the SNARE proteins for membrane fusion, only little is known about the key biochemical steps that exocyst stimulates in the course of SNARE complex assembly, a critical question defining the essential molecular role of the exocyst complex. Here, we use a combination of single molecule and bulk fluorescence assays with purified octameric yeast exocyst complexes to examine the role of exocyst in a reconstituted SNARE assembly and fusion system. We show that the exocyst complex simulates multiple steps spanning from SNARE protein activation to ternary complex assembly, rather than affecting only a specific subset of steps. We also observed that the exocyst has important downstream roles in driving membrane fusion, up to full content mixing of vesicle lumens. Our results suggest that the exocyst complex provides extensive chaperoning functions for the entire process of SNARE complex assembly, presumably using its multi-faceted structure provided by the eight subunits.Competing Interest StatementThe authors have declared no competing interest.