RT Journal Article
SR Electronic
T1 A novel mechanism for left-right asymmetry establishment involving tissue remodeling and MyoID
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2022.01.16.476383
DO 10.1101/2022.01.16.476383
A1 Lefèvre, Bénédicte M.
A1 Delvigne, Marine
A1 Vidal, Josué
A1 Courtier-Orgogozo, Virginie
A1 Lang, Michael
YR 2022
UL http://biorxiv.org/content/early/2022/01/17/2022.01.16.476383.abstract
AB How novel left-right asymmetries evolve in animals is unknown. Drosophila pachea males display a unique left-right asymmetry in the genital organs and mate in a right-sided position onto the female. In flies, male genitalia undergo a clockwise 360° rotation during development. To test if this tissue remodeling process is linked to asymmetry development in the male genitalia of Drosophila pachea, we developed CRIPSR and transgenesis in this singular species. As in D. melanogaster, mutants of the MyoID gene homolog undergo reverse rotation of the developing genitalia. In addition, they have mirror-image asymmetric genitalia. Although their genital asymmetry is reversed mutants still adopt a right-sided copulation posture. Our results reveal a novel mechanism for establishing left-right asymmetry in animals, involving complex tissue remodeling and MyoID.One Sentence Summary D. pachea genital left-right size asymmetry is established through MyoID-mediated tissue remodelling and does not determine the direction of mating posture.Competing Interest StatementThe authors have declared no competing interest.