PT  - JOURNAL ARTICLE
AU  - Rhiannon M. Sears
AU  - Kyle J. Roux
TI  - Mechanisms of A-type lamin targeting to nuclear ruptures are disrupted in <em>LMNA</em>- and <em>BANF1</em>-associated progerias
AID  - 10.1101/2022.01.14.476371
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.01.14.476371
4099  - http://biorxiv.org/content/early/2022/01/18/2022.01.14.476371.short
4100  - http://biorxiv.org/content/early/2022/01/18/2022.01.14.476371.full
AB  - Mutations in the genes LMNA and BANF1 can lead to accelerated aging syndromes called progeria. The protein products of these genes, A-type lamins and BAF, respectively, are nuclear envelope (NE) proteins that interact and participate in various cellular processes, including nuclear envelope rupture and repair. BAF localizes to sites of nuclear rupture and recruits NE-repair machinery, including the LEM-domain proteins, ESCRT-III complex, A-type lamins, and membranes. Here, we show that it is a mobile, nucleoplasmic population of A-type lamins that is rapidly recruited to ruptures in a BAF-dependent manner via BAF’s association with the Ig-like β fold domain of A-type lamins. These initially mobile lamins become progressively stabilized at the site of rupture. Farnesylated prelamin A and lamin B1 fail to localize to nuclear ruptures, unless that farnesylation is inhibited. Progeria-associated LMNA mutations inhibit the recruitment affected A-type lamin to nuclear ruptures, due to either permanent farnesylation or inhibition of BAF binding. A progeria-associated BAF mutant targets to nuclear ruptures but is unable to recruit A-type lamins. Together, these data reveal the mechanisms that determine how lamins respond to nuclear ruptures and how progeric mutations of LMNA and BANF1 impair recruitment of A-type lamins to nuclear ruptures.Competing Interest StatementThe authors have declared no competing interest.