PT  - JOURNAL ARTICLE
AU  - Dmitry Lapin
AU  - Viera Kovacova
AU  - Xinhua Sun
AU  - Joram Dongus
AU  - Deepak D. Bhandari
AU  - Patrick von Born
AU  - Jaqueline Bautor
AU  - Nina Guarneri
AU  - Johannes Stuttmann
AU  - Andreas Beyer
AU  - Jane E. Parker
TI  - A coevolved EDS1-SAG101-NRG1 module mediates cell death signaling by TIR-domain immune receptors
AID  - 10.1101/572826
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 572826
4099  - http://biorxiv.org/content/early/2019/03/10/572826.short
4100  - http://biorxiv.org/content/early/2019/03/10/572826.full
AB  - Plant intracellular nucleotide-binding/leucine-rich repeat (NLR) immune receptors are activated by pathogen effectors to trigger host defenses and cell death. Toll-Interleukin1-receptor (TIR)-domain NLRs (TNLs) converge on the Enhanced Disease Susceptibility1 (EDS1) family of lipase-like proteins for all resistance outputs. In Arabidopsis TNL immunity, AtEDS1 heterodimers with Phytoalexin Deficient4 (AtPAD4) transcriptionally boost basal defense pathways. AtEDS1 uses the same surface to interact with PAD4-related Senescence-Associated Gene101 (AtSAG101), but the role of AtEDS1-AtSAG101 heterodimers was unclear. We show that AtEDS1-AtSAG101 function together with AtNRG1 coiled-coil domain helper NLRs as a coevolved TNL cell death signaling module. AtEDS1-AtSAG101-AtNRG1 cell death activity is transferable to the solanaceous species, Nicotiana benthamiana, and cannot be substituted by AtEDS1-AtPAD4 with AtNRG1 or AtEDS1-AtSAG101 with endogenous NbNRG1. Analysis of EDS1-family evolutionary rate variation and heterodimer structure-guided phenotyping of AtEDS1 variants or AtPAD4-AtSAG101 chimeras identify closely aligned α-helical coil surfaces in the AtEDS1-AtSAG101 partner C-terminal domains that are necessary for TNL cell death signaling. Our data suggest that TNL-triggered cell death and pathogen growth restriction are determined by distinctive features of EDS1-SAG101 and EDS1-PAD4 complexes and that these signaling machineries coevolved with further components within plant species or clades to regulate downstream pathways in TNL immunity.