PT  - JOURNAL ARTICLE
AU  - Sammari, Malika
AU  - Inglebert, Yanis
AU  - Ankri, Norbert
AU  - Russier, Michaël
AU  - Incontro, Salvatore
AU  - Debanne, Dominique
TI  - Synaptic and intrinsic potentiation in O-LM interneurons is induced by theta patterns of stimulation
AID  - 10.1101/2022.01.19.476905
DP  - 2022 Jan 01
TA  - bioRxiv
PG  - 2022.01.19.476905
4099  - http://biorxiv.org/content/early/2022/01/21/2022.01.19.476905.short
4100  - http://biorxiv.org/content/early/2022/01/21/2022.01.19.476905.full
AB  - Oriens lacunosum-moleculare (O-LM) interneurons display a non-conventional form of long-term synaptic potentiation (LTP) conferred by calcium-permeable AMPA receptors (CP-AMPAR). So far, this form of LTP has been induced in O-LM cells by physiologically unrealistic protocols. We report here the induction of both synaptic and intrinsic potentiation in O-LM interneurons following stimulation of afferent glutamatergic inputs in the theta (θ) frequency range. LTP is induced by synaptic activation of CP-AMPAR whereas long-term potentiation of intrinsic excitability (LTP-IE) results from the mGluR1-dependent down-regulation of Kv7 voltage-dependent potassium channel and hyperpolarization activated and cyclic nucleotide-gated (HCN) channel through the depletion of phosphatidylinositol-4,5-bi-phosphate (PIP2). LTP and LTP-IE are reversible, demonstrating that both synaptic and intrinsic changes are bidirectional in O-LM cells. We conclude that physiological stimuli such as θ patterns induce synaptic and intrinsic potentiation in O-LM interneurons.Competing Interest StatementThe authors have declared no competing interest.