TY - JOUR T1 - GLH/VASA helicases promote germ granule formation to ensure the fidelity of piRNA-mediated transcriptome surveillance JF - bioRxiv DO - 10.1101/2022.01.21.477267 SP - 2022.01.21.477267 AU - Wenjun Chen AU - Jordan S Brown AU - Tao He AU - Wei-Sheng Wu AU - Shikui Tu AU - Zhiping Weng AU - Donglei Zhang AU - Heng-Chi Lee Y1 - 2022/01/01 UR - http://biorxiv.org/content/early/2022/01/22/2022.01.21.477267.abstract N2 - The ability to distinguish non-self from self is the key characteristic for any defense system. piRNAs function as guardians of the genome by silencing non- self nucleic acids and transposable elements in animals. Many piRNA factors are enriched in perinuclear germ granules, but whether their localization is required for piRNA biogenesis or function is not known. Here we show that GLH/VASA helicase mutants exhibit defects in forming perinuclear condensates containing PIWI and other small RNA cofactors. These mutant animals produce largely normal levels of piRNA but are defective in triggering piRNA silencing. Strikingly, while many piRNA targets are activated in GLH mutants, we observed that hundreds of endogenous genes are aberrantly silenced by piRNAs. This defect in self versus non-self recognition was also observed in other mutants where perinuclear P granules are disrupted. Together, our results argue that perinuclear germ granules function critically to promote the fidelity of piRNA- based transcriptome surveillance in C. elegans and preserve self versus non-self distinction.Competing Interest StatementThe authors have declared no competing interest. ER -